Hydrocarbondegrading microorganisms are ubiquitous in the marine environment 27, 76, and biodegradation. Frontiers petroleum hydrocarbondegrading bacteria for. These strains grow by using a limited number of organic compounds, including the aromatic hydrocarbons naphthalene, phenanthrene, anthracene, and toluene, as sole carbon sources. The function of hydrocarbon degrading bacteria mainly depends on their hydrocarbon degrading enzymes, the expression and activity of which are closely related to the physiological activity of bacteria mukherjee et al. Hydrocarbons, enrichment culture technique, oil degrading bacteria, gravimetric analysis, biochemical tests. The observation that the oceans are not covered with an oily layer is a testimony to the activity of the hydrocarbon. An unexpected enrichment for hydrocarbon degrading bacteria, along with an increase in the abundance and transcription of hydrocarbon degrading genes, was found at depths near the trench bottom 10,400m and 10,500 m. One of the most important features of hydrocarbon degrading bacteria is the ability.
Microbial biodegradation is the use of bioremediation and biotransformation methods to harness the naturally occurring ability of microbial xenobiotic metabolism to degrade, transform or accumulate environmental pollutants, including hydrocarbons e. Among the true marine marinobacter genus alteromonadaceae family associated with n alkane transformation, there are several strains metabolizing both saturated. Comparative studies on bacterial consortia for hydrocarbon. Identification of hydrocarbon degrading bacteria the bacteria isolated were identified based on physical characterization and the biochemical tests outlined in bergeys manual of determination bacteriology holt et al. Furthermore, the dominant petroleum hydrocarbon degrading bacteria were shifted from arcobacter in production water to pseudomonas after the first subculture and then to bacillus after the sixth subculture. The present study describes the isolation and identification of total heterotrophic bacterial thb population and hydrocarbon degrading bacterial hdb population, identification of the efficient degrader, its molecular characterization and phylogenetic analysis based upon 16s rna gene sequencing from the samples collected in ennore creek. Consequently, microorganisms with pahdegradation ability and soil fertility improvement attributes are sought after in order to achieve sustainable remediation of polluted sites. Isolation of hydrocarbon degrading bacteria from soils. Hydrocarbons are generally colorless and hydrophobic with only weak odors. Petroleum hydrocarbon degrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Bacteria displaying such capabilities are often exploited for the. The optimum ph for hydrocarbon degradation by flavobacterium spp. Isolation and identification of effective hydrocarbon. Succession of hydrocarbondegrading bacteria in the.
Alcanivorax borkumensis was the first hcb to have its genome sequenced. The heteroatom groups in the crude oil were biodegraded simultaneously with normal alkanes and alkyl cyclohexanes. Pdf polycyclic aromatic hydrocarbons pahs are of particular concern due. Because of their diverse molecular structures, it is difficult. Aitken department of environmental sciences and engineering, school of public health, university of north carolina at chapel hill, chapel hill, north carolina 275997400 received 9 december 1999accepted 6 march 2000. The occurrence of polycyclic aromatic hydrocarbons pah in refinery effluents is of great concern globally due to its persistence, recalcitrance and carcinogenicity. Isolation and characterization of hydrocarbon degrading bacteria from environmental habitats in western new york state, masters thesis in chemistry, 2005.
Distribution of hydrocarbondegrading bacteria in the soil. This study isolated pahdegrading bacteria from enriched cultures of spent automobile engineoil. They had been reported as organisms capable of anaerobic degradation of pxylene and nalkanes, respectively. Hydrocarbon oxidizing bacteria have been isolated from a variety of terrestrial and aquatic environments, using both enrichment and direct plating techniques.
International journal of current microbiology and applied. Many indigenous microorganisms in water and soil are capable of degrading. Crude oil and petroleum is a complex mixture of hydrocarbons, which cause widespread environmental contaminants following a spill. Reconstructing metabolic pathways of hydrocarbondegrading. Results the result of the bacterial count showed 38. Medina hydrocarbon degrading bacteria consists of known nonpathogenic microbial strains that degrade petroleum hydrocarbons in solid and aqueous environments. Microbial degradation of hydrocarbons in the environment.
Contaminated soil samples were collected from warri refinery jetty while water sample was collected from. In the same sand samples for which detailed chemical analysis was performed, mpn counts of cultivatable hydrocarbondegrading bacteria in oilcontaminated sands 2. Floodgate 36 listed 25 genera of hydrocarbon degrading bacteria and 25 genera of hydrocarbon degrading fungi which were isolated. Pdf screening of hydrocarbon degrading bacteria isolated. Succession of hydrocarbondegrading bacteria in the aftermath. For these facultative hydrocarbon degraders the occasional supertanker oil spill forms an occasional carbon banquet. Isolation and molecular identification of hydrocarbon. Bacteria are the most active agents in petroleum degradation, and they work as primary degraders of spilled oil in environment 37, 38. Petroleum hydrocarbons can be degraded by microorganisms such as bacteria, fungi, yeast and microalgae 3, 4. Botanyenvironmental pollution,mbamarketing green apple environmental technologies 287patpadganj industrial area, delhi110 092india brief facts bacteria generally degraders in aquatic systems such as oceans, ponds and other water reservoir. Sufficient time is needed to synthesize hydrocarbon degrading enzymes because of the requirements of.
Petroleum hydrocarbon degrading bacteria were obtained after enrichment on crude oil as a chocolate mousse in a continuous supply of indonesian seawater amended with nitrogen, phosphorus and iron nutrients. Proliferation of hydrocarbondegrading microbes at the bottom. Study on the biodegradation of crude oil by free and. Comparative study of five polycyclic aromatic hydrocarbon degrading bacterial strains isolated from contaminated soils. Pdf isolation and screening of hydrocarbon degrading. Draft genome sequences of oysterassociated pseudomonas stutzeri strain mf28, p. Sample collection sediment samples were collected from the munakkal beach trichur dist, kerala located on the west coast of india immediately after the major event tsunami in 2004.
The principal hydrocarbon degrading microorganisms are bacteria and fungi. Viability of hydrocarbondegrading bacterial consortium immobilized on different carriers was studied. With the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has increased, resulting in an urgent need for remediation. Aug 17, 2017 at present, many types of hydrocarbon degrading bacteria that can utilise different types of hydrocarbons as carbon and energy sources in metabolism exist in the marine environment 1, 2. Potential of polycyclic aromatic hydrocarbondegrading. Dec 03, 2018 petroleum hydrocarbon degrading bacteria. We describe the carbonization of these clays using the sustainable biopolymer, chitosan, to optimize wetting. Restoration of polycyclic aromatic hydrocarbon pah polluted sites is presently a major challenge in agroforestry. Microbiological nitrogen fixation is an activity that requires large amounts of energy. Pdf isolation, identification and characterization of. Twentythree hydrocarbondegrading bacteria strains were isolated from gas station leakingcontaminated groundwater. Bioremediation of petroleum based contaminants with biosurfactant. Isolation and identification of hydrocarbon degrading. Enrichment and isolation of petroleum hydrocarbon degrading bacteria petroleum hydrocarbon degrading bacteria were isolated from oil contaminated soil as described above.
Department of microbiology, rishi biotech, mumbai400053, india corresponding author a b s t r a c t experiments were performed in low and high 1% and 10% concentration of crude bacillus introduction. Microorganisms and culture conditions petroleum hydrocarbon degrading bacteria, bacillus cereus s1, was isolated from oilpolluted sea. They also possess diverse metabolic pathways which is not seen in fungi which allows them. Powtoon is a free tool that allows you to develop cool animated clips and animated presentations for your website, office meeting, sales pitch, nonprofit fundraiser, product launch, video resume. Characterization of aromatic hydrocarbon rading bacteria. Pdf with the sharp increase in population and modernization of. Wholegenome sequences of five oysterassociated bacteria.
The in situ metabolism of oildegrading bacteria is likely to be limited by a number of environmental parameters, including temperature, the availability of oxygen and major nutrients, oil hydrocarbon content, and weathering or dispersal of the oil. Bacteria that cause for biodegradable plastic usually are the dominant hydrocarbon degraders in landfill environments. Hydrocarbondegrading microbial communities with high tolerance to and high degrading ability of. Isolation of hydrocarbon degrading bacteria following the strategy designed in our work to isolate hydrocarbon degrading bacteria both with high growth and biological activity, 39 purified bacterial isolates were recorded amongst hundreds of isolated ones at the first step of the program. Microbial degradation of petroleum hydrocarbon contaminants. Genomewide surveys of these isolates suggest that the oyster microbiome, which remains largely understudied, has a strong potential to degrade. Media used in this study were mineral salts medium msm, which contained per liter. This study was aimed at bacterial degradation of polycyclic aromatic hydrocarbons pah in untreated refinery effluent.
Torrent semiconductor sequencing, dna nanoball sequencing, heliscope single. Pdf characterization of hydrocarbon degrading bacteria. Isolation and identification of hydrocarbon degradation bacteria. Aliphatic hydrocarbons decreased in comparison with those of the abiotic control regarding the nonresolved background cycloalkanes, resins and asphaltenes. Hydrocarbon degrading bacteria are important resources for use in phytoremediation applications. Petroleum hydrocarbondegrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Anaerobic hydrocarbondegrading sulfatereducing bacteria.
Petroleum hydrocarbondegrading bacteria for the remediation. Immobilization was by direct adsorption of the isolates. The hydrocarbon degrading bacterial types and numbers were found to be lesser than their heterotrophic counterparts. Anaerobic degradation of oil hydrocarbons by sulfatereducing. Most petroleum hydrocarbons encountered in the environment are ultimately degraded or metabolized by indigenous bacteria because of their energetic and carbon needs for growth and reproduction, as well as the requirement to relieve physiological stress caused by the presence of petroleum hydrocarbons in the microbial bulk environment hazen et al.
During screening for biosurfactantproducing, nalkane degrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea watersediment samples collected near the isle of borkum north sea, using mihagols c14,15nalkanes as principal carbon source. Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with. Hydrocarbon degrading bacteria medina agriculture products. Isolation, screening and activity of hydrocarbondegrading. Characterization of hydrocarbon degrading bacteria isolated from hydrocarbon contaminated soil. Hydrocarbondegrading bacteria and the bacterial community. An unexpected enrichment for hydrocarbondegrading bacteria, along with an increase in the abundance and transcription of hydrocarbondegrading genes, was found at depths near the trench bottom 10,400m and 10,500 m. Screening and isolation of a hydrocarbon degrading. Source of isolation the source for bacterial isolation consisted of wastewaters contaminated with used motor oil, diesel and lubricating oils. Aerobic bacteria degrading both n alkanes and aromatic. The biodegradation of crude oil in soil by immobilized cells was compared with that by free cells at three.
This bacterium is of particular interest as it is a cosmopolitan marine bacterium that blooms in oil. Feb 06, 20 the present study describes the isolation and identification of total heterotrophic bacterial thb population and hydrocarbon degrading bacterial hdb population, identification of the efficient degrader, its molecular characterization and phylogenetic analysis based upon 16s rna gene sequencing from the samples collected in ennore creek. Similarly, in preliminary study 57 oil degrading bacteria were isolated from oil impregnated soil in search of potent hydrocarbon degrader 14. Genomic diversity of two hydrocarbondegrading and plant. Pdf characterization of hydrocarbon degrading bacteria isolated. Hydrocarbons are degraded primarily by bacteria and fungi. Several bacteria are even known to feed exclusively on hydrocarbons yakimov et al. Kleindienst s 2012 hydrocarbondegrading sulfatereducing bacteria in marine hydrocarbon seep sediments. Incubation of the isolated nonvirulent strains in bushnellhaas bh medium supplemented with hydrocarbon, showed their ability to survive on hydrocarbon thereby reducing the total organic.
Pdf identification of hydrocarbon degrading bacteria by. Isolation and characterization of hydrocarbon degrading. Individually immobilized and surfacemodified hydrocarbon. This study aimed to isolate and identify hydrocarbon degrading bacteria and. The level of petroleum hydrocarbon degradation was determined by gravimetric assay at each 5 days interval. Bacteria generally degraders in aquatic systems such as oceans, ponds and other water reservoir. Petroleum hydrocarbondegrading bacteria and the type of petroleum. The goal of this project was to evaluate the effects of test conditions and bacterial growth conditions on the results and precision of the math test using a single species of hydrocarbon degrading bacteria. In previous studies, two hydrocarbon degrading sulfatereducing bacteria, strains pp31 t and pl12 t, were obtained from oilpolluted marine sediments of shuaiba, kuwait.
We tracked succession of plume bacteria before, during and after the 83day spill to determine the microbial response and biodegradation potential throughout the incident. Pdf petroleum hydrocarbondegrading bacteria for the. Hydrocarbons from which one hydrogen atom has been removed are functional groups called hydrocarbyls. Temperatures in this subtropical environment remain optimal for microbial growth throughout the summer, and oxygen profiling indicates that the pensacola beach sands remained aerobic throughout the study period with oxygen concentrations at all. Enrichment cultures containing naphthalene, phenanthrene, fluoranthene, or pyrene as a sole carbon and energy source were monitored by. Interest in possible hydrocarbon oxidation by sulfatereducing bacteria arose from their frequent presence in oil production. Department of chemistry, rochester institute of technology, rochester, ny. Viability of hydrocarbondegrading bacterial consortium. Products from the incomplete metabolism of pyrene by. Diversity of diesel degrading bacteria from a hydrocarbon. Hydrocarbondegrading bacteria were isolated from 2% hydrocarbon enriched sample collected from vehicular repairing stations, garages and petrol bunk.
These aerobic, gramnegative rods are motile by means of. Marine oil spill bioremediation by immobilized and free cells. Particle stabilized emulsions provide an environmentally friendly alternative to the chemical dispersion of oil in the event of a spill over water. Adaptation is brought about by selective enrichment of hydrocarbonutilizing microorganisms and amplification of the pool of hydrocarboncatabolizing genes. They were related to alcanivorax and marinobacter strains, which are ubiquitous petroleum hydrocarbon degrading bacteria in marine environments, and to oceanobacter kriegii 96. The indigenous hdb were widely distributed throughout the soil environment and ranged from 3. The highest and lowest bacterial counts in topsoil samples were found out to be 2. Hydrocarbondegrading bacteria were isolated from crude oil contaminated sites in gio and kdera, rivers state, nigeria using enrichment method. Three heterotrophic bacterial strains were isolated from different locations in puget sound, washington, by using biphenyl as the principal carbon source. Biodegradation is a cost effective and ecofriendly treatment for oilcontaminated materials by the use of microorganisms.
Isolation and characterization of hydrocarbondegrading. Isolation and screening of hydrocarbon degrading bacteria from soil near kadi gujarat region. Hydrocarbondegrading bacteria enriched by the deepwater horizon oil spill identified by cultivation and dnasip. Green,1,3 gina freyer,1,2 andy canion, jonathan delgardio, 1nikita. They were resistant to heavy metals such as lead, iron, zinc, cobalt and mercury. Hydrocarbon degrading bacteria are known as hydrocarbonoclastic bacteria hcb. Pdf table of contents submit biotechnology research international.
Screening of hydrocarbon degrading bacteria isolated from oil contaminated soil article pdf available june 2019 with 192 reads how we measure reads. The diversity of indigenous bacteria in sediments from several sites in the elizabeth river virginia able to degrade multiple polycyclic aromatic hydrocarbons pahs was investigated by the use of classical selective enrichment and molecular analyses. Supplied in a dry bran mixture, microorganisms are activated when mixed with water. To characterize polycyclic aromatic hydrocarbon pah. Pdf oceanobacterrelated bacteria are important for the. Hydrocarbondegrading bacteria are important resources for use in phytoremediation applications. Yet, for many hydrocarbonoclastic strains the genetic information regarding pollutant degradation and detoxification has not been thoroughly revealed. These isolates were purified from soil samples on the basis of their colony morphology, texture, growth, temperature and hydrocarbon utilization ability. Enrichment, isolation, and phylogenetic identification of. Ringim mahi saidu, gumel ahmad mohammed, shiaka gimba peter, isolation and molecular identification of hydrocarbon degrading bacteria from contaminated soil in mechanic village dutse, jigawa state, international journal of microbiology and biotechnology. The isolated hydrocarbon degrading bacterial strains were analyzed with gram staining, motility test, spore. Biodegradation of crude oil in contaminated soils by free and. Mineral clay particles present in abundance in marine environments adsorb at oilwater interfaces forming stable emulsion droplets. Also, samples amended with cp were found to have more types and higher numbers of heterotrophic and hydrocarbon degrading bacteria than the samples without amendment controls.
Adaptation by prior exposure of microbial communities to hydrocarbons increases hydrocarbon degradation rates. However, cultures have not been preserved, or attempts to reproduce hydrocarbon oxidation by desulfovibrio strains failed 1, 2. Necessity of consortium with broad enzymatic capacities for degradation of complex hydrocarbon pollutants such as crude oil or diesel fuel was also reported. Aromatic hydrocarbon degrading bacteria chikoma kazunga and michael d. Department of microbiology, rishi biotech, mumbai400053, india corresponding author a b s t r a c t experiments were performed in low and high 1% and 10% concentration of. Microbes behind the scenes of the biodegradability of plastic. Hundred milliliters of the cell free media was shaken for foam formation and.
Despite its toxicity, a considerable fraction of petroleum oil entering marine systems is eliminated by the hydrocarbondegrading activities of microbial communities, in particular by a recently discovered group of specialists, the hydrocarbonoclastic bacteria hcb. In the present study, we demonstrated that hydrocarbonoclastic bacteria isolated from all over kuwait exhibited diazotrophic potential, as well hydrocrbonoclastic actual. These gramnegative, aerobic, rodshaped bacteria use a limited number of organic. Screening and isolation of a hydrocarbon degrading bacteria by enrichment culture technique. The isolation of hydrocarbon degrading bacteria in topsoil and subsoil samples of selected mechanic workshops located in the benin metropolis was carried out. The deepwater horizon oil spill produced large subsurface plumes of dispersed oil and gas in the gulf of mexico that stimulated growth of psychrophilic, hydrocarbon degrading bacteria. Based on this information, we experimentally tested and confirmed the hydrocarbon degradation activity of. Although bacteria able to grow on aliphatic and aromatic hydrocarbons are found in many genera, the genera alcanivorax appear to be special because these bacteria are specialized for. Simultaneous degradation of atrazine and phenol by pseudomonas sp.
Jyothi kommula, vit university, for the award of the degree of master of science, is a record of bonafide work carried. Isolation and identification of effective hydrocarbon degrading bacteria from diesel polluted soil doi. One potent gram negative bacillus bacteria was isolated on the basis of petroleum hydrocarbon degradation efficiency and identified as pseudomonas sp. Aromatic hydrocarbons such as benzene, hexane, toluene, naphthalene and xylene degrading bacteria such as flavobacterium spp. Proliferation of hydrocarbondegrading microbes at the. Isolation and characterization of hydrocarbondegrading bacteria. In organic chemistry, a hydrocarbon is an organic compound consisting entirely of hydrogen and carbon 620 hydrocarbons are examples of group 14 hydrides. Enrichment, isolation and identification of hydrocarbon degrading bacteria udgire m. Fifty seven 57 bacteria degrading crude oil from the hydrocarbon polluted sites of gasoline and diesel spilled gas stations of coimbatore, india have been isolated. Altogen labs has developed active bioremediation technology based on natural oildegrading bacteria for largescale remediation of hydrocarboncontaminated water and soil. Hydrocarbondegrading bacteria and the bacterial community response in gulf of mexico beach sands impacted by the deepwater horizon oil spill joel e. Several bacteria are even known to feed exclusively on hydrocarbons 39. During screening for biosurfactantproducing, nalkanedegrading marine bacteria, six heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from sea watersediment samples collected near the isle of borkum north sea, using mihagols c14,15nalkanes as principal carbon source.
Proximate analyses were carried out on the best carrier materials. The hydrocarbo n degrading bacterial counts for the. Genes free fulltext genomic diversity of two hydrocarbon. Directional culture of petroleum hydrocarbon degrading. Engineered clays as sustainable oil dispersants in the. The bacterial strains were isolated by inoculating the soil and water sample into the prepared nutrient agar medium which is autoclaved and added 1.
Pnp degrading bacteria and this item was submitted in to occur in microform, scholars, gejun. For these facultative hydrocarbon degraders the occasional supertanker oil spill forms. Frontiers petroleum hydrocarbondegrading bacteria for the. They also posses diverse metabolic pathways that are not present in fungi which allows them to utilize most recalcitrant petroleum hydrocarbons. Isolation and identification of petroleum hydrocarbon. Petroleum hydrocarbondegrading bacteria for the remediation of. Characteristic assessment of dieseldegrading bacteria. Sep 14, 2016 powtoon is a free tool that allows you to develop cool animated clips and animated presentations for your website, office meeting, sales pitch, nonprofit fundraiser, product launch, video resume. Jun, 2018 interestingly, marine hydrocarbon degrading bacteria belonging to gammaproteobacteria also revealed the broadened preferences towards structurally divergent groups of hydrocarbons. A realtime pcr quantification method for indigenous hydrocarbondegrading bacteria hdb carrying the alkb gene in the soil environment was developed to investigate their distribution in soil.
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